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Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis
Enables detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent
Brand: Invitrogen™ C10445
Includes: Five single-use vials with enough total reagent for five 96-well plates or 100 coverslips, and cumene hydroperoxide as a positive control
Description
This kit localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from approx. 590nm to approx. 510nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry.
- Includes cumene hydroperoxide as positive control compound to induce lipid peroxidation in cells
- Reagent is provided as stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
- Form: Frozen
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Nitro-Oxidative Stress, Reactive Oxygen Species
Order Info
Shipping Condition: Wet Ice
Specifications
| Green | |
| Frozen | |
| 1 kit |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Image-iT Lipid Peroxidation Kit, for live cell analysis
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 2
- Aspiration toxicity Category 1
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Organic peroxide Type E & F
- Skin corrosion/irritation Category 1 B
- Specific target organ toxicity after repeated exposure Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H242-Heating may cause a fire.
- H302-Harmful if swallowed.
- H304-May be fatal if swallowed and enters airways.
- H312-Harmful in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H319-Causes serious eye irritation.
- H331-Toxic if inhaled.
- H335-May cause respiratory irritation.
- H351-Suspected of causing cancer.
- H373-May cause damage to organs.
- H411-Toxic to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : Cumene hydroperoxide, Dimethyl sulfoxide
Certificates
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Product Title
Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis > 1 kit
For Research Use Only. Not for use in diagnostic procedures.
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