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Invitrogen™ Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate Product Code.: 11554267

Invitrogen™ Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate

Product Code. 11554267
100 μg, 100µg
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Quantity:
100 μg
500 μg
Unit Size:
100µg
500µg
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Product Code. 11554267

Brand: Invitrogen™ C34776

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This item is not returnable. View return policy

Made from a recombinant version of the B subunit only to provide a very high-purity product that is completely free of the toxic A subunit

  • Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside GM1, making it powerful tool for retrograde labeling of neurons
  • This tracer has been used in variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall
  • When used in neuronal tracing applications, CT-B is typically introduced by pressure injection or by iontophoretic injection into neural tissue
  • At neutral pH, 11.4 kDa B subunit exists as 57 kDa pentamer
  • Lyophilized product can be dissolved in buffer (e.g., PBS) for use
  • More recently, researchers have found that CT-B can be used as marker for lipid rafts, which are membrane microdomains enriched in cholesterol and sphingolipids thought to be important in cell signaling
  • For lipid raft staining, cells are first incubated with fluorescent CT-B, then an anti-CT-B antibody is added to crosslink the CT-B in the lipid rafts into distinct patches on the plasma membrane
  • These patches are easily visualized by fluorescence microscopy

  • Label (Ex/Em): Alexa Fluor™ 555 (555/565nm)
  • At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer

Cell Analysis, Cell Structure, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Lipid Rafts, Microbial Tracking, Neuronal Tracing

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Quantity 100 μg
Conjugate Alexa Fluor 555
Recombinant Recombinant
Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Label Type Alexa Fluor Dyes
Shipping Condition Room Temperature
Protein Form Recombinant
Form Lyophilized
Protein Subtype Cholera Toxin
Product Line Alexa Fluor™

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2916405 Certificate TypeCertificate of Analysis Date24/05/2024 Product CodeC34776
Lot Number2680433 Certificate TypeCertificate of Analysis Date14/07/2023 Product CodeC34776
Lot Number2552977 Certificate TypeCertificate of Analysis Date23/03/2023 Product CodeC34776
Lot Number2442122 Certificate TypeCertificate of Analysis Date03/02/2022 Product CodeC34776
Lot Number2310126 Certificate TypeCertificate of Analysis Date03/06/2021 Product CodeC34776

For Research Use Only. Not for use in diagnostic procedures.

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