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Thermo Scientific™ Phosphorylating Phosphoramidite, standard grade, serum vial bottle
Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)).
Brand: Thermo Scientific™ 27-1794-04
Additional Details : Weight : 0.01420kg
Description
Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 5 mL amber bottle with a serum vial neck style, which is compatible with most Applied Biosystems synthesizers and AKTA synthesizers of oligonucleotide synthesizers.
Features:
• Full chemical name: 2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula: C34H45F3N2O7PS
• Formula weight: 656.66 g/mol
• HPLC Purity: ≥95.0%
Specifications
AKTA Synthesizers, Applied Biosystems Synthesizers | |
Store in freezer (at or below -20°C). | |
Room Temperature | |
0.25 g | |
Standard | |
Powder |
Phosphorylating CEP | |
None (Modifier) | |
95% | |
Phosphoramidite | |
None (Modifier) |