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Invitrogen™ Human MMP3 Matched Antibody Pair

Artikelnummer. 10184603
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Mængde:
10 x 96 Tests
Pakningsstørrelse:
Hver
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Artikelnummer. 10184603 Leverandør Invitrogen™ Leverandørnr. CHC1543
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Forventet forsendelse: 18-08-2026
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Beskrivelse

ELISA

The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P08254
Analyseområde 1.25-20 ng/mL
Assay følsomhed <1.25 ng/mL
Konjugeret HRP
Genadgangsnr. P08254
Gene Alias CHDS6, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1, CHDS6; EC 3.4.24.17; EMS-2; matrix metallo protease; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 precursor; matrix metalloproteinase-3; metalloproteinase 3 receptor; MMP; MMP10; MMP3; MMP-3; MMP-3 protein; MMPs; progelatinase; proteoglycanase; SL-1; SLN1; SLN-1; STMY; STMY1; Str1; STR-1; stromelysin; stromelysin 1; Stromelysin1; stromelysin-1; Transin1; Transin-1CHDS6; EC 3.4.24.17; EMS-2; matrix metallo protease; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 precursor; matrix metalloproteinase-3; metalloproteinase 3 receptor; MMP; MMP10; MMP3; MMP-3; MMP-3 protein; MMPs; progelatinase; proteoglycanase; SL-1; SLN1; SLN-1; STMY; STMY1; Str1; STR-1; stromelysin; stromelysin 1; Stromelysin1; stromelysin-1; Transin1; Transin-1; CHDS6; MMP-3; SL-1; STMY; STMY1; STR1
Gensymboler MMP3
Immunoassay-kitformat Matched Antibody Pair
Produkttype ELISA, ELISA Kit
Mængde 10 x 96 Tests
Prøvetype Plasma, Serum, Supernatant
Regulatorisk status RUO
Substrattype Proteases
Gen-id (Entrez) 4314
Målarter Human
Til brug med (udstyr) Absorbance Microplate Reader, Colorimetric Microplate Reader
Inkluderer Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Indhold og opbevaring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gen symbol MMP3
Sættets indhold Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etiket eller farvestof Biotin
Protein aliaser SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1
Forsendelsestilstand Wet Ice
Opbevaringskrav 2-8°C
Mål MMP3
Test tid 1 hr 30 min
Samlet analysetid 24 hr
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