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Invitrogen™ MegaScript™ T7 Transcription Kit Plus

Artikelnummer. 31662351
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31662351 25 reaktioner
17984681 50 reaktioner
17934741 1000 reaktioner
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Artikelnummer. 31662351 Leverandør Invitrogen™ Leverandørnr. A5762225

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Beskrivelse

De MegaScript T7 Transcription Kit Plus indeholder alle de reagenser og buffere, der er nødvendige for in vitro-syntese af store mængder RNA.

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein. Easily scale from RUO to GMP with the incorporation of TheraPure reagents, eliminating reoptimization during scale up.

Features of the MegaScript T7 Transcription Kit Plus include:

  • Optimized to achieve high mRNA yields (>5 mg/mL)
  • Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
  • Includes GFP control template
  • Made up of high quality reagents (TheraPure); eliminates the need to qualify new reagents during scale-up
  • Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

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Tekniske data

Celletype Primary Cells, Stem Cells, iPSC, Established Cell Lines, Hard-to-Transfect Cells
Endelig produkttype In vitro Transcribed RNA
Format Kit
Isoleringsteknologi Silica Spin Column
Oprensningsmål In vitro Transcribed RNA
Prøvetype In vitro Transcribed RNA, Total RNA
Antal reaktioner 25 reaktioner
Produkttype IVT mRNA kit
Mængde 25 Reactions

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