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Tekniske data
Tekniske data
| Til brug med (applikation) | This product has been tested by SDS-PAGE. Multi-purpose MDA-MB-231 Whole Cell Lysate are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95C for 5 minutes. Sample is ready for use in immunoprecipitation and ELISA experiments, conditions should be optimized by the user. It is recommended to use TrueBlot IP reagents for immunoprecipitation experiments. |
| Mængde | 500 μg |
| Indhold og opbevaring | Store at -70C. Avoid freeze-thaw cycles. |
| Mål | MDA-MB-231 |
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