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Thermo Scientific™ MabCaptureC™ High Capacity Protein A Resin
Beskrivelse
GoPure MabCaptureC Screening Plates, 96-well, 50 μL, contain MabCaptureC High-Capacity Protein A Resin, a high-performance chromatography resin for capturing monoclonal antibodies (mAbs) and recombinant Fc-tagged fusion proteins. It provides high specific-binding capacity and enhanced alkaline-stability characteristics under clean-in-place conditions (CIP), for more robust purification and resin regeneration. These ready-to-use, 96-well spin plates offer a high-throughput format for rapid antibody purification, screening, and evaluating multiple conditions in a single plate experiment.
Features of MabCaptureC High Capacity Protein A Resin
- High binding capacity—60 mg hIgG/mL at 5-minite residence (80 mg/mL at 10-minute residence)
- Alkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH, enabling efficient cleaning of the chromatography resin and improving product quality
- Animal component-free raw materials
- Versatility—clean antibody or Fc-fusion protein preparations from cell culture supernatant, serum, or ascites
- Stability—high-affinity Protein A is securely immobilized to high purity agarose to prevent leaching of ligand, target antibody, or base support
- Flexible— available in multiple formats, including bulk loose resin, spin columns, and 96-well spin plates
The Protein A ligand, which is produced in yeast, is immobilized on a rigid and uniformly sized agarose bead with an average particle size of 75 μm. The uniformity of the particle size, in turn, delivers superior performance characteristics over traditional resins.
MabCaptureC High-Capacity Protein A Resin, alkaline-stable, is suitable for the purification of mAbs as well as a subpopulation of scFv's and Fab fragments that contain a VH3 domain.
Available formats
- Resin slurries—10 mL, 50 mL, and 200 mL of 50% slurry
- Spin columns—10 mL spin columns containing 1 mL of settled resin, supplied at 5 mL 20% slurry
- GoPure 96-well spin plates—96-well spin plates with 20 μL or 50 μL of settled resin
Tekniske data
Tekniske data
| Produkttype | Spin Column |
| Kolonnetype | Agarose Resin, Affinity |
| Format | Spin Column |
| Stationær fase | Protein A |
| Mængde | 5 Columns |
| Forsendelsestilstand | Wet Ice |
Må kun bruges til undersøgelsesformål. Må ikke anvendes ved diagnostiske procedurer.
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