missing translation for 'onlineSavingsMsg'
Learn More

Ly-6G Rat anti-Mouse, Functional Grade, Clone: 1A8-Ly6g, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9668-82

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15507836

  • 1128.00 DKK / 100µg

Estimated Shipment
15-04-2021

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown. Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity. Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis and depletion of neutrophils. Moreover, this antibody has been reported to have antagonist activity. (Fluorochrome-conjugated 1A8-Ly6g is recommended for use in flow cytometry.). Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.
Specifications

Specifications

Ly-6G
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
P35461
Gr-1, Gr1, Ly-6G
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry, Functional Assay
1A8-Ly6g
Functional Grade
Ly6g
Liquid
Ly6g
IgG2a, kappa
100 μg
4° C
Primary
546644
Videos
SDS
Documents

For Research Use Only.