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Thermo Scientific™ Luminaris Color Probe qPCR Master Mix

Optimize qPCR with probe detection using standard cycling protocols with these ready-to-use master mixes with premixed UDG, available with or without blue color.

2030.00 DKK - 30350.00 DKK

Specifications

Description Color Probe
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
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Products 4
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
13273169
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Thermo Scientific™
K0353
1,250 Reactions
9380.00 DKK
1250 reactions
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Estimated Shipment: 10-04-2024
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13293169
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Thermo Scientific™
K0351
250 Reactions
2030.00 DKK
250 reactions
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Estimated Shipment: 22-04-2024
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13283169
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Thermo Scientific™
K0354
5,000 Reactions
30350.00 DKK
5000 reactions
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Estimated Shipment: 22-04-2024
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13263169
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Thermo Scientific™
K0352
500 reactions
3920.00 DKK
500 reactions
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Estimated Shipment: 22-04-2024
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Description

Description

Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

Highlights

  • Blue Master Mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance
    • Specificity — Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
    • Sensitivitity — single copy detection
    • Wide linear range — accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience — ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX for different real-time platforms
  • Standard cycling protocols
Specifications

Specifications

Color Probe
Tube
qPCR
Standard
Luminaris
Gene Expression
DNA, dsDNA
Primer-probe
High
Taq DNA Polymerase
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
qPCR Master Mix
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.