Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, low ROX
Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.
Brand: Thermo Scientific™ K0344
Additional Details : Weight : 0.22000kg
Thermo Scientific™ Luminaris Color Probe qPCR Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.Thermo Scientific Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.
The use of Luminaris Color Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.
Specificity no primer dimers
Sensitivitity detects low copy number targets
Wide linear range accurate quantification across 9 orders of magnitude
Reproducibility and convenience ready-to-use 2X master mix
- Blue Master Mix and Yellow Sample Buffer for easy pipetting
- UDG in the master mix to prevent carry-over contamination
- Excellent qPCR performance
- Versions with premixed ROX for different real-time platforms
- Standard cycling protocols
- Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye
- 40X Yellow Sample Buffer with a yellow dye
- Nuclease-free water
Gene expression analysis; siRNA validation; Genotyping; Pathogen detection
|Color Probe Low ROX|
|Taq DNA Polymerase|
|4 x 12.5 mL|
|7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P|
|5000 x 20µL rxns|
|2X (Luminaris Color Probe Low ROX qPCR Master Mix), 40X (Yellow Sample Buffer)|
|Real Time PCR (qPCR)|
|qPCR Master Mix|
|5000 Reactions of 20 μL|