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Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, high ROX

Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

2000.00 DKK - 29330.00 DKK

Specifications

Description Color Probe High ROX
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
13273159
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Thermo Scientific™
K0331
250 x 20 μL Reactions
2000.00 DKK
250 reactions
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Estimated Shipment: 11-04-2024
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13283159
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Thermo Scientific™
K0332
500 x 20 μL Reactions
3895.00 DKK
500 reactions
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Estimated Shipment: 22-04-2024
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13203169
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Thermo Scientific™
K0333
1250 x 20 μL Reactions
9230.00 DKK
1250 reactions
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Estimated Shipment: 22-04-2024
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13213169
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Thermo Scientific™
K0334
5000 x 20 μL Reactions
29330.00 DKK
5000 reactions
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Estimated Shipment: 22-04-2024
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Description

Description

Thermo Scientific™ Luminaris Color Probe qPCR Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.

Thermo Scientific Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms.

  • Specificity no primer dimers

  • Sensitivitity detects low copy number targets

  • Wide linear range accurate quantification across 9 orders of magnitude

  • Reproducibility and convenience ready-to-use 2X master mix

  • Blue Master Mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance
  • Versions with premixed ROX for different real-time platforms
  • Standard cycling protocols
Luminaris Color Probe High ROX qPCR Master Mix (2X) and Luminaris Color Probe Low ROX qPCR Master Mix (2X) contain different amounts of ROX passive reference dye for specific real-time platforms.

Includes:

  • Luminaris Color Probe qPCR Master Mixes include Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye
  • 40X Yellow Sample Buffer with a yellow dye
  • Nuclease-free water

Recommended for:

Gene expression analysis; siRNA validation; Genotyping; Pathogen detection

Specifications

Specifications

Color Probe High ROX
Tube
qPCR
Standard
Luminaris
Gene Expression, Genotyping, siRNA Validation
DNA, dsDNA
Primer-probe
High
Taq DNA Polymerase
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
qPCR Master Mix
2X (Luminaris Color Probe High ROX qPCR Master Mix), 40X (Yellow Sample Buffer)
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.