Invitrogen™ Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6)

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Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. Recommended positive controls: IHC (P) - Human tonsil and testis tissue, common marmoset spleen tissue, rat esophagus, small intestine and liver tissue, mouse embryonic skin tissue IHC (F) - Rat lymph node tissue, transgenic mouse spinal cord tissue ICC/IF - HAP1 cells, human cardiac stem cells, HEp-2 cells, rat cardiomyocytes Flow - HAP1 cells.

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.