Tocris Bioscience™ Janelia Fluor™ 552, Haloalkane

Application: live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). In vivo functional imaging of Ca2+ responses (flies/mice); multiplexed functional imaging of neurons and astrocytes in zebrafish larvae; quantification of Ca2+ concentration using fluorescence lifetime imaging microscopy (FLIM).

Properties and Photophysical Data: Stable to fixation. Cell permeable. KD = 87 ? 5. In the presence of EGTA (Ca2+ chelator) excitation and emission maxima (λ) are 561 nm and 559-578 nm, respectively; quantum yield = 0.05; extinction coefficient = 73,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); brigthness = 3.6 mM-1cm-1. In the presence of saturating Ca2+, excitation and emission maxima (λ) are 562 nm and 565-582 nm, respectively; quantum yield = 0.25; extinction coefficient = 78,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); brigthness = 19.5 mM-1cm-1.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.