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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

3140.00 DKK - 9290.00 DKK

Specifications

Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
Cloning Method Blunt TOPO™
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
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Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10256812
View Documents Promotion Details
Invitrogen™
K280020
25 Reactions
5620.00 DKK
Each
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10584033
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Invitrogen™
K280040
50 Reactions
9290.00 DKK
Each
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15375533
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Invitrogen™
K2800J10
10 Reactions
3140.00 DKK
10 reactions
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Description

Description

  • Each kit has the Zero Blunt™ TOPO™ vector containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants
  • Available with a variety of competent cells, or without competent cells
  • Fast and easy—go from PCR to clones in just 3 steps, including a 5-minute cloning reaction
  • Efficient—obtain up to 95% clones with correct insert
  • Proven—enabling reliable performance for over a decade with over 20,000 citations
  • Simple —no ligase, post-PCR procedures, or PCR primers containing specific sequences required
  • Accurate—for use with high fidelity DNA polymerases, no need for A-overhang on PCR product
  • The pCR™Blunt II-TOPO™ vector is designed for cloning of blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum™ Pfx DNA Polymerase.
  • Allows for direct selection of recombinants via disruption of the lethal E. coli gene ccdB. The vector contains the ccdB gene fused to the C-terminus of the LacZα fragment. Ligation of a blunt-end PCR product disrupts expression of the LacZα-ccdB gene fusion, permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.
  • Using TOPO™ cloning technology, there is no need for PCR primers containing specific sequences, post-PCR procedures, vector preparation, or other time-intensive DNA manipulation steps. Just add your PCR reaction straight to the provided topoisomerase-charged vector, incubate 5 minutes, and transform E. coli competent cells.
  • Zero Blunt™ TOPO™ PCR Cloning kits for direct insertion of blunt-ended PCR products into a plasmid vector can be purchased with a variety of competent cells that deliver different advantages

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

TOP10
Blunt TOPO™
Kit
SP6
Chemically Competent
Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
PCR Cloning Kit
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.