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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

For fast, efficient cloning and subsequent in vitro transcription/translation

Brand:  Invitrogen™ K4600J10

Additional Details : Weight : 0.25000kg

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Product Code. 15380204

  • 2615.00 DKK / 10 reactions

In stock, ready to ship: 1

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Description

Description

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This kit includes the pCR™II-TOPO TA vector.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies

Applications:

Cloning, PCR Cloning

Specifications

Specifications

Ampicillin (AmpR), Kanamycin (KanR)
TOPO™-TA
TOPO-TA Vectors
TOP10
TOPO™
10 reactions
Videos
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.