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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli Product Code.: 10573263
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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli

Product Code. 10573263
40 Reactions, pCR2.1, Each
Click to view available options
No. of Reactions:
20 Reactions
40 Reactions
Vector:
pCR2.1
pCRII
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10573263

Brand: Invitrogen™ K200040

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This item is not returnable. View return policy

Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a 15-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of PCR product
  • Streamlined: does not require use of PCR primers that contain restriction sites
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing

  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to the 3' ends of PCR products
  • Linearized vector supplied in has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning

TRUSTED_SUSTAINABILITY

Specifications

Bacterial or Yeast Strain INVαF ́
Cell Type Chemically Competent E. coli
Cloning Method TA Cloning
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
For Use With (Application) PCR Cloning
No. of Reactions 40 Reactions
Product Line One Shot™
Product Type Cloning Kit
Promoter T7
Quantity 40 reactions
Vector pCR2.1
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Product Identifier
  • TA Cloning Kit, with pCR 2.1 Vector and One Shot INV
Signal Word
  • Warning
Hazard Category
  • Reproductive toxicity Category 2
  • Skin sensitiser Category 1
Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H361-Suspected of damaging fertility or the unborn child.
Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
  • MIXTURE LIST-Contains : 2-Mercaptoethanol, Hydrogen chloride

For Research Use Only. Not for use in diagnostic procedures.

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