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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli
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No. of Reactions:
20 Reactions
40 Reactions
Vector:
pCR2.1
pCRII
Unit Size:
Each
Description
The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a 15-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 promoter for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to the 3' ends of PCR products
- Linearized vector supplied in has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
Cloning, PCR Cloning

Specifications
Specifications
Bacterial or Yeast Strain | INVαF ́ |
Cell Type | Chemically Competent E. coli |
Cloning Method | TA Cloning |
Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
For Use With (Application) | PCR Cloning |
No. of Reactions | 40 Reactions |
Product Line | One Shot™ |
Product Type | Cloning Kit |
Promoter | T7 |
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Safety and Handling


Product Identifier
- TA Cloning Kit, with pCR 2.1 Vector and One Shot INV
- Warning
- Reproductive toxicity Category 2
- Skin sensitiser Category 1
- H317-May cause an allergic skin reaction.
- H361-Suspected of damaging fertility or the unborn child.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 2-Mercaptoethanol, Hydrogen chloride
For Research Use Only. Not for use in diagnostic procedures.
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Product Title
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli >
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