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Invitrogen™ T-REx™ Complete Kit, with pcDNA™4/TO© Vector
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Mængde:
1 kit
Pakningsstørrelse:
5g
Beskrivelse
Indeholder
20μg of pcDNA4/TO or 20μg each of pcDNA4/TO/myc-His A, B, and C; a positive control vector, pcDNA;6/TR, and forward and reverse sequencing primers plus 1g Zeocin (100mg/mL), 50mg of Blasticidin, and 5g of tetracycline. All vectors are supercoiled and provided lyophilized.
- Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known
- Specific Activation: uses a repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline
- Because elements do not use viral transactivators, you can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes
- T-REx transcriptional control elements are provided through two tetracycline operator sequences (TetO2) that have been inserted between TATA box of CMV promoter and transcriptional start site
- TetO2 sequence itself has no effect on expression
- When the tetracycline repressor protein (TR) is present, it effectively binds TetO2 sites and blocks transcription initiation
- Tetracycline added to culture medium binds to, and changes conformation of, TR protein; This change causes TR protein to release TetO2 sites, derepressing transcription from CMV promoter
- Result is high-level expression of gene of interest
- Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
- T-REx is a powerful inducible mammalian expression system that allows to regulate expression from complete human cytomegalovirus (CMV) enhancer-promoter
- Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO2 sequence for high-level regulated expression
- Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines
- Large multiple cloning site to simplify cloning
- In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody
- Regulatory vector, pcDNA™6/TR, is provided for high-level expression of the tetracycline repressor (TR) protein
- Expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein
Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression
Tekniske data
Tekniske data
| Konstitutivt eller inducerbart system | Inducible |
| Leveringstype | Transfection |
| Inducerende middel | Tetracycline |
| Promotor | CMV/TO |
| Produkttype | T-Rex Complete Cloning Kit with Vector |
| Udvælgelsesagent (eukaryot) | Zeocin™, Blasticidin |
| Indhold og opbevaring | The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO, a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers. The T-REx™ Complete Kit includes the Core Kit plus 1 g Zeocin™ (100 mg/ml), 50 mg of Blasticidin, and 5 g of tetracycline. All vectors are supercoiled and provided lyophilized. Store all components at -20°C. Zeocin™ and tetracycline should be protected from exposure to light. All components are guaranteed stable for 6 months when properly stored. |
| Protein tag | Untagged |
| Kloningsmetode | Restriction Enzyme/MCS |
| Mængde | 1 kit |
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For Research Use Only. Not for use in diagnostic procedures.
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