Learn More
Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour.
Brand: Invitrogen™ K210026
Additional Details : Weight : 0.50000kg
Description
Transfection grade. The PureLink HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA. Using HiPure Filter Columns and HiPure Precipitators supplied in kit, you can purify plasmid DNA without centrifugation steps required in traditional isolation protocols. The quality of purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA.
- Simple protocols—bacterial lysate clarification and isopropanol precipitation without centrifugation
- Fast results—from E. coli cell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
- High yields—up to 850μg from a high-copy plasmid
Specifications
Filter Column | |
Column | |
Bacterial Culture | |
Plasmid DNA | |
Not High-throughput Compatible (Manual) | |
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC | |
PureLink™ | |
10 Preps | |
Room Temperature | |
PureLink™ | |
1000 μg |
The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature. | |
Anion Exchange Resin | |
15 mL | |
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
10 Preps | |
> 200 μg (Large-Scale) Plasmid DNA | |
Plasmid FP MaxiPrep Kit | |
Transfection Grade | |
Plasmid DNA | |
1 hr. |
Safety and Handling
- HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
Supplemental information
- MIXTURE LIST-Contains : Sodium lauryl sulphate, Sodium hydroxide, Sodium hydroxide
For Research Use Only. Not for use in diagnostic procedures.