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Invitrogen™ pPICZ A, B, & C Pichia Vectors

Designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein

Brand:  Invitrogen™ V19020

Product Code. 17124629

  • 9190.00 DKK / 20µg

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Includes: The EasySelect PichiaExpression Kit includes 20μg each of pPICZ A, B, and C and pPICZ A, B, and C, Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression, 250mg Zeocin , media (YP and YNB), EasyComp PichiaTransformation Kit, 2μg each 5' AOX1, 3' AOX1,and α-factor sequencing primers. A lacZ positive expression control is provided with pPICZ.
This item is not returnable. View return policy

Description

Description

  • These vectors contain Zeocin™ resistance gene for direct selection of multi-copy integrant strains
  • By selecting with increasing amounts of Zeocin, strains with multiple copies of the gene of interest integrated into genome are obtained
  • Increasing number of copies of gene of interest in recombinant Pichiastrain can result in higher expression levels
  • Inducible AOX1promoter for high-level expression in Pichia pastoris c-mycepitope tag for convenient detection with Anti-mycAntibody
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • The pPICZ vector also features secretion signal for efficient transport of proteins to medium

Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression

TRUSTED_SUSTAINABILITY
Specifications

Specifications

AOX1
20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.

Store vectors at -5 to -30°C.
His Tag (6x), c-Myc Epitope Tag
20 μg
Pichia Expression Vector
Zeocin™ (ZeoR)
Restriction Enzyme/MCS
pPIC
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Invitrogen™ pPICZ A, B, & C Pichia Vectors > 20μg

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