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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli

Product Code. 10286662
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Quantity:
20 Reactions
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Includes

TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

  • Fast, 5-minute TOPO TA Cloning delivers 95% recombinants
  • 3'-T overhangs simplify cloning of Taq-amplified PCR products
  • Fast growing MachI cells shorten cloning time
  • Sequencing primer sites located close to cloning insertion site so sequences more insert, less vector first time
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

TRUSTED_SUSTAINABILITY

Specifications

Cloning Method TOPO™ with Gateway™
Content And Storage The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored
No. of Reactions 20 reactions
Product Line One Shot™
Product Type TA Cloning Kit
Quantity 20 Reactions
Vector pCR

For Research Use Only. Not for use in diagnostic procedures.

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