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Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit
Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3.
Brand: Invitrogen™ 11363D
Additional Details : Weight : 0.11000kg
Includes: 2mL Antibody Mix Human CD4, 10mL Depletion MyOne; Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1 x 109 cells
Description
Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.
Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)
Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™
Starting sample
PBMC
Order Info
Shipping Condition: Room Temperature
Specifications
| T Cells (Regulatory) | |
| Cell Isolation Kit | |
| Processes ∼1 x 109 cells total | |
| DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™ | |
| PBMC | |
| 1 x 108 PBMCs per isolation |
| Each Kit Contains: • 2 mL Antibody Mix Human CD4 • 10 mL Depletion MyOne™ Dynabeads™ • 5 mL Dynabeads™ CD25 • 2 mL DETACHaBEAD™. These reagents are sufficient to process ∼1 x 109 cells. Store components at 2°C to 8°C. |
|
| Depletion, then positive isolation | |
| >95% | |
| Research Grade | |
| Room Temperature | |
| Human |
For Research Use Only. Not for use in diagnostic procedures.