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Invitrogen™ ChargeSwitch™ gDNA Mini Bacteria Kit
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Artikelnummer. 10319402
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Mængde:
50 Preps
Pakningsstørrelse:
Hver
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Artikelnummer. 10319402

Brand: Invitrogen™ CS11301

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Beskrivelse

Indeholder

  • ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
  • ChargeSwitch Resuspension Buffer (R4)
  • ChargeSwitch Lysis Buffer (L14)
  • ChargeSwitch Binding Buffer (B8)
  • ChargeSwitch Wash Buffer (W12)
  • ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
  • Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)

PCR, restriction enzyme digestion, cloning, Southern blotting, and sequencing downstream applications

The ChargeSwitch gDNA Mini Bacteria Kit uses magnetic bead-based technology to provide rapid and efficient purification of up to 12μg of genomic DNA from up to 0.5mL of Gram-positive or Gram-negative bacterial culture or an individual colony in less than 15 minutes after lysate preparation

  • Optimized for higher-purity DNA extraction
  • Improved performance with accompanying magnetic rack
  • Cleaner purified plasmid for optimal downstream performance
  • High quality, high yield plasmid DNA
  • Compatible with MagnaRack™ magnetic rack
  • Improved performance in downstream applications
  • Less hazardous, less waste

DNA & RNA Purification & Analysis, DNA Extraction, Genomic DNA Purification, gDNA from Bacteria

Ordreinfo

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Tekniske data

Indhold og opbevaring All components of the ChargeSwitch™ gDNA (genomic DNA) Mini Bacteria Kit are shipped at room temperature. Upon receipt, store components as follows: Store Proteinase K at 4“C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:

ChargeSwitch™ Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch™ Resuspension Buffer (R4)
ChargeSwitch™ Lysis Buffer (L14)
ChargeSwitch™ Binding Buffer (B8)
ChargeSwitch™ Wash Buffer (W12)
ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
Format Kit
Isoleringsteknologi ChargeSwitch Chemistry
Skala Mini
Prøvetype Bacteria
Til brug med (applikation) PCR, Southern Blotting, Sequencing, Cloning
High-throughput kompatibilitet Not High-throughput Compatible (Manual)
Produktlinje ChargeSwitch
Produkttype gDNA Mini Bacteria Kit
Mængde 50 Preps
Forsendelsestilstand Room Temperature
Mål Genomic DNA
Test tid 15 min
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For Research Use Only. Not for use in diagnostic procedures.

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