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Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase

Brand:  Invitrogen™ 12344024

Additional Details : Weight : 0.00454kg

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Product Code. 10472482

  • 3060.00 DKK / Each

In stock, ready to ship: 14

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Description

Description

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

  • Achieve greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplify fragments up to 12 kb
  • Minimize PCR optimization
  • Built-in hot start

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Specifications

Specifications

3' - 5'
AccuPrime™
Approved for shipment on Wet or Dry Ice
26 X
200 Reactions
AccuPrime™ Pfx DNA Polymerase
Standard
100 μL
Built-In Hot Start
200 reactions
Primer-Probe
Low
Blunt
Separate Components
12 kb or less
Videos
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.