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Thermo Scientific™ Imject™ EDC mcKLH Spin Kit

Artikelnummer. 11803855 Shop alle Thermo Scientific produkter
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Mængde:
5 x 2 mg
Pakningsstørrelse:
1 sæt
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11803855 5 x 2 mg 1 sæt
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Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.
Artikelnummer. 11803855 Leverandør Thermo Scientific™ Leverandørnr. 77671
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Denne vare kan ikke returneres. Se returpolicy
Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Description

Prepare immunogens (hapten-carrier conjugates) with your antigen of choice and mariculture KLH using different conjugation chemistries.

Thermo Scientific™ Imject Mariculture Keyhole Limpet Hemocyanin (mcKLH) is formulated to achieve optimum performance with different conjugation chemistries, and includes a complete kit for carbodiimide-mediated hapten-carrier preparation.

Mariculture KLH (mcKLH) is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation. The large, complex protein contains hundreds of sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers.

Preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of KLH carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.

Highlights:

  • Convenient packages – choose lyophilized KLH protein or complete EDC conjugation kit

  • High-yield conjugation – each molecule of KLH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers

  • Validated quality – purified and stabilized mariculture KLH maintains solubility in aqueous solutions, unlike traditional sources of the carrier protein

  • Sustainable source – KLH is harvested from select populations of the mollusk Megathura crenulata (keyhole limpet) that are grown in mariculture, not extracted from wild populations

  • Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin

TRUSTED_SUSTAINABILITY

Specifications

Indhold og opbevaring Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide
• Imject Mariculture KLH (in MES buffer), 5 x 2 mg
• Imject EDC Conjugation Buffer, 30 mL
• EDC Crosslinker, 5 x 10 mg
• Imject Purification Buffer Salts, 5 x 0.84 g
• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columns

Store at 4°C.
Oversigt Imject EDC mcKLH Spin Kit
Format Kit
Mængde 5 x 2 mg
Produktlinje Imject

For Research Use Only. Not for use in diagnostic procedures.

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