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Invitrogen™ Human IL1RA ELISA Kit

Artikelnummer. 10610354
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Mængde:
96 prøver
Pakningsstørrelse:
96 tests
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Artikelnummer. 10610354 Leverandør Invitrogen™ Leverandørnr. KAC1181
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Beskrivelse

ELISA

The Human IL-1RA ELISA Kit quantifies human Interleukin 1 Receptor Antagonist (IL-1RA) in serum, plasma, buffered solution, urine or cell culture medium. The assay will recognize both the natural and recombinant IL-1RA. Principle of the method The Human IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P18510
Analyseområde 31,2 til 2000 pg/ml
Assay følsomhed 4 pg/mL
Indhold og opbevaring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Konjugeret HRP
Immunoassay-kitformat Sandwich ELISA Kit
Produkttype ELISA
Protein familie Cytokines & Receptors
Prøvetype Plasma, Serum, Supernatant, Urin
Målarter ADN
Til brug med (applikation) Kolorimetrisk
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias Anakinra; CD121A; cytokine; D2S1473; DIRA; F630041P17Rik; ICIL-1RA; IL 1 receptor antagonist; IL1 inhibitor; il1 receptor; il-1 receptor; IL1 receptor antagonist; IL1F3; IL1R; il-1r; IL1RA; IL-1RA; IL1-RA; IL-1ra3; IL-1R-alpha; IL1RN; IL-1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; interleukin-1 receptor; interleukin-1 receptor antagonist; interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist secretory form; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; IRAP1; MGC10430; MVCD4; P80; type II interleukin-1 receptor antagonistAnakinra; CD121A; cytokine; D2S1473; DIRA; F630041P17Rik; ICIL-1RA; IL 1 receptor antagonist; IL1 inhibitor; il1 receptor; il-1 receptor; IL1 receptor antagonist; IL1F3; IL1R; il-1r; IL1RA; IL-1RA; IL1-RA; IL-1ra3; IL-1R-alpha; IL1RN; IL-1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; interleukin-1 receptor; interleukin-1 receptor antagonist; interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist secretory form; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; IRAP1; MGC10430; MVCD4; P80; type II interleukin-1 receptor antagonist; DIRA; ICIL-1RA; IL-1RN; IL-1ra; IL-1ra3; IL1F3; IL1RA; IRAP; MVCD4
Gen-id (Entrez) 3557
Gen symbol DIRA,ICIL-1 RA,IL-1 RN,IL-1 ra,IL-1 ra3,IL1F3,IL1RA,IRAP,MVCD4
Interassay CV 6.1%
Intraassay CV 4.5%
Sættets indhold Forbelagt 96-brønds plade, Standard, Standardfortyndingsbuffer, Inkubationsbuffer, Biotinyleret detektionsantistof, Streptavidin-HRP, HRP fortynder, Vaskebuffer, kromogen, Stop Løsning, Kontrolelementer
Etiket eller farvestof Biotin
Præcision 4 pg/mL
Protein aliaser IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor
Mængde 96 prøver
Regulatorisk status RUO
Prøvevolumen Plasma 100μ L, Serum 100μ L, Supernatant 100μ L, Urin 10μ L
Forsendelsestilstand Wet Ice
Opbevaringskrav 2 °C til 8 °C
Mål IL1RA
Test tid 1 t 20 min
Samlet analysetid 3 timer 30 min
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