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IL-10 Rat anti-Human, Biotin, Clone: JES3-12G8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-7109-85

Additional Details : Weight : 0.25000kg

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Product Code. 15380030

  • 1972.00 DKK / 500µg

In stock, ready to ship: 3

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Description

Description

Description: The JES3-12G8 antibody reacts with human interleukin-10 (IL-10). Applications Reported: The JES3-12G8 antibody has been reported for use in the detection of human IL-10 by ELISA. Applications Tested: The JES3-12G8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-10 (IL-10) in combination with the purified JES3-9D7 (14-7108) antibody for capture and recombit human IL-10 (14-8109) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
Specifications

Specifications

IL-10
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P22301
Interleukin-10, CSIF, IL-10, IL10A, TGIF
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
ELISA
JES3-12G8
Biotin
IL10
Liquid
IL10
IgG2a, kappa
500 μg
4° C, store in dark, DO NOT FREEZE!
Primary
3586
Videos
SDS
Documents

For Research Use Only.