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Invitrogen™ Human IL-10 Matched Antibody Pair

Artikelnummer. 10775153
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Mængde:
10 x 96 Tests
Pakningsstørrelse:
Hver
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10775153 10 x 96 Tests Hver
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Artikelnummer. 10775153 Leverandør Invitrogen™ Leverandørnr. CHC1323
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Forventet forsendelse: 07-08-2026
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Beskrivelse

ELISA

The Human IL-10 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-10. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P22301
Analyseområde 31.2-2000 pg/mL
Assay følsomhed 31.2 pg/mL
Konjugeret HRP
Genadgangsnr. P22301
Gene Alias CSIF, GVHDS, IL-10, IL10A, MGC126450, MGC126451, TGIF, CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; GVHDS; IL-10A; TGIF; IL10
Gensymboler IL10
Immunoassay-kitformat Matched Antibody Pair
Produkttype ELISA, ELISA Kit
Mængde 10 x 96 Tests
Prøvetype Plasma, Serum, Supernatant
Gen-id (Entrez) 3586
Målarter Human
Til brug med (udstyr) Absorbance Microplate Reader, Colorimetric Microplate Reader
Inkluderer Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Indhold og opbevaring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gen symbol IL10
Sættets indhold Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etiket eller farvestof Biotin
Protein aliaser IL-10, Cytokine synthesis inhibitory factor, CSIF
Regulatorisk status RUO
Forsendelsestilstand Wet Ice
Opbevaringskrav 2-8°C
Mål IL-10
Test tid 1 hr 30 min
Samlet analysetid 24 hr
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