Rat IgG2b kappa, PerCP-eFluor™ 710, clone: eB149/10H5, Isotype Control, eBioscience™
Rat Isotype Control
Brand: Affymetrix eBioscience 46-4031-82
Additional Details : Weight : 0.25000kg
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: PerCP-eFluor™ 710 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: PerCP-eFluor™ 710 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells.Use at the same concentration as experimental antibody.PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Rat IgG2b kappa|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IgG2, IgG2b, IgG2bk|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Control, Flow Cytometry|
For Research Use Only.