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Mouse IgG2b kappa, PerCP-eFluor™ 710, clone: eBMG2b, Isotype Control, eBioscience™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 46-4732-80

Additional Details : Weight : 0.25000kg

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Product Code. 15381320

  • POA / 25µg

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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: Mouse IgG2b Isotype Control PerCP-eFluor™ 710 has been reported for use in flow cytometric analysis.Applications Tested: Mouse IgG2b K Isotype Control PerCP-eFluor™ 710 has been tested by flow ctometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG2b kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2b, IgG2bk
IgG2b, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBMG2b
PerCP-eFluor 710
Liquid
Murine
Affinity chromatography
RUO
Isotype Control
Not Applicable
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