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Invitrogen™ Mus IGF-1 ELISA Kit

Artikelnummer. 15435283
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Mængde:
10 x 96 tests
5 x 96 prøver
96 prøver
Pakningsstørrelse:
1 sæt
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Artikelnummer. Mængde Pakningsstørrelse
15435283 96 prøver 1 sæt
17135739 5 x 96 prøver -
18597774 10 x 96 tests -
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Artikelnummer. 15435283 Leverandør Invitrogen™ Leverandørnr. EMIGF1
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Beskrivelse

ELISA

Musens insulinlignende vækstfaktor 1 (Ms IGF-1) ELISA kvantificerer Ms IGF-1 i museserum, plasma eller cellekulturmedium. Analysen vil udelukkende genkende både naturlig og rekombinant Ms IGF-1. Metodens princip The Mouse IGF-1 solid-phase sandwich ELISA (enzym-linked immunosorbent assay) er designet til at måle mængden af målet bundet mellem et matchet antistofpar. Et målspecifikt antistof er blevet præcoatet i brøndene på den medfølgende mikroplade. Prøver, standarder eller kontroller tilsættes derefter til disse brønde og binder til det immobiliserede (fangende) antistof. Sandwichen dannes ved tilsætning af det andet (detektor) antistof, en substratopløsning tilsættes, der reagerer med enzym-antistof-målkomplekset for at producere et målbart signal. Intensiteten af dette signal er direkte proportional med koncentrationen af mål til stede i den originale prøve. Strenge validering Hvert fremstillet parti af dette ELISA kit er kvalitetstestet for kriterier som sensitivitet, specificitet, præcision og lot-to-lot konsistens. Se manualen for mere information om validering.

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P05017
Analyseområde 2,74 til 2000 pg/ml
Assay følsomhed 4 pg/mL
Kalibreringsområde 4 pg/ml til 2000 pg/ml
Indhold og opbevaring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Fastfase sandwich ELISA
Konjugeret HRP
Immunoassay-kitformat Sandwich ELISA Kit
Produkttype ELISA
Protein familie Vækstfaktorer& Receptorer
Prøvetype Plasma, Serum, Supernatant
Specificitet Denne ELISA kit viser ingen krydsreaktivitet med følgende testede cytokiner: Mus CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 beta,, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-M, M, FIP, M, F, 1, M, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Tilstrækkelig til 96 analyser
Målarter Mus
Til brug med (applikation) ELISA, Proteinanalyser, Proteinbiologi, Kræftbiologi, Immunologi, Neurovidenskab, Neurobiologi, Proteinpåvisning, Proteinanalyse
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias C730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-CC730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-C; C730016P09Rik; Igf-1; Igf-I
Gen-id (Entrez) 16000
Gen symbol C730016P09Rik,Igf-1,Igf-I
Interassay CV <12%
Intraassay CV <10%
Sættets indhold Forbelagt 96-brønds plade, Standard, Assay Fortynder koncentrat, Biotinyleret detektionsantistof, SAV-HRP, Vaskebuffer, kromogen, Stop Løsning, Klæbende pladedæksler
Etiket eller farvestof Biotin
Protein aliaser IGF-I, Somatomedin
Mængde 96 prøver
Regulatorisk status RUO
Forskningsdisciplin Udvikling
Prøvevolumen Plasma 1μ L, Serum 1μ L, Supernatant 100μ L
Forsendelsestilstand Wet or Dry Ice
Opbevaringskrav 2 °C til 8 °C
Synonym C730016P09Rik, Igf-1, Igf-I
Mål IGF1
Test tid 1 t 20 min
Samlet analysetid 4 timer 45 min
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