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IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
1854.00 DKK
Specifications
Antigen | IFN gamma |
---|---|
Clone | XMG1.2 |
Concentration | 0.5 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Description
Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity. Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
IFN gamma Monoclonal antibody specifically detects IFN gamma in Mouse samples. It is validated for Flow Cytometry.Specifications
IFN gamma | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P01580 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
XMG1.2 | |
Flow Cytometry | |
Alexa Fluor 488 | |
Rat | |
Mouse | |
15978 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |
For Research Use Only.