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Invitrogen™ Human IFN gamma Ucoated ELISA Kit

Artikelnummer. 15541107
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Mængde:
10 x 96 tests
20 x 96 tests
Pakningsstørrelse:
Pakke med 10
Pakke med 20
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15541107 20 x 96 tests Pakke med 20
15561107 10 x 96 tests Pakke med 10
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Artikelnummer. 15541107 Leverandør Invitrogen™ Leverandørnr. 88731677
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Beskrivelse

ELISA

Human Interferon gamma (IFNγ ) Ubelagt ELISA Sættet indeholder præ-matchede antistofpar og reagenser til udførelse af kvantitative enzymforbundne immunosorbentanalyser (ELISA ) for at påvise og kvantificere proteinniveauer af humant IFNγ . Vaskebuffer og stopopløsning er nødvendige for at fuldføre ELISA reaktion og sælges separat. Metodens princip ELISA'er er designet til at måle mængden af målet bundet mellem et matchet antistofpar. Et målspecifikt antistof er coatet til bunden af brøndene på en mikroplade, hvilket er en proces natten over. Prøver, standarder eller kontroller tilsættes derefter til disse brønde og binder til det immobiliserede (fangende) antistof. En sandwich dannes ved tilsætning af det andet (detektor) antistof, en substratopløsning tilsættes, der reagerer med enzym-antistof-målkomplekset for at producere et målbart signal. Intensiteten af dette signal er direkte proportional med koncentrationen af mål til stede i den originale prøve.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P01579
Analyseområde 4 til 500 pg/ml
Assay følsomhed 8 pg/mL
Indhold og opbevaring Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme: Pre-titrated Streptavidin-HRP
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Konjugeret HRP
Immunoassay-kitformat Uncoated ELISA Kit
Produkttype ELISA
Prøvetype Plasma, Serum, Supernatant
Målarter ADN
Til brug med (applikation) ELISA
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-gBoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g; IL-12p35; Il-12a; Ll12a; p35;Il-12b; Il-12p40; Il12p40; p40
Gen-id (Entrez) 3458
Gen symbol IL-12p35, Il-12 A, Ll12a, p35, Il-12 b, Il-12p40, Il12p40, p40
Sættets indhold Capture Antibody: Fortitreret, Oprenset antistof, Detektionsantistof: Fortitreret, Biotin-konjugeret antistof, Standard: Rekombinant protein til generering af standardkurve og kalibrering af prøver, 10X Coating Buffer: Buffer til plettering af Capture Antibody, 5X ELISA/ELISPOT Fortynder: Buffer til blokering og fortynding af detektionsantistoffet og enzymet, Enzym: Præ-titreret Avidin-HRP, Underlag: 1X TMB-løsning, Analysecertifikat: Lot-specifikke instruktioner til fortynding af antistoffer og standarder, 96-brøndsplade: Højaffinitetsplade med 96 brønde (inkluderet med produktkatalognr., der ender på suffikserne -22, -76, -86)
Etiket eller farvestof Biotin
Protein aliaser IFN-gamma, Immune interferon
Mængde 20 x 96 tests
Regulatorisk status RUO
Prøvevolumen Plasma 100μ L, Serum 100μ L, Supernatant 100μ L
Opbevaringskrav 2 °C til 8 °C
Synonym Interferon-gamma, IFN-g, IFNg
Mål IFN gamma
Test tid 1 t 45 min
Samlet analysetid 24 timer 30 min
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