IFN gamma, APC-eFluor™ 780, clone: XMG1.2, eBioscience™
Rat Monoclonal Antibody
Brand: Invitrogen 47731180
Additional Details : Weight : 0.00010kg
DescriptionDescription: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.06μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor™ 780 emits at 780nm and is excited with the Red laser (633nm). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647nm; Emission: 780nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|4°C, store in dark, DO NOT FREEZE!|