Invitrogen™ iBlot™ 2 Starter Kit

The iBlot 2 Starter Kit includes a iBlot 2 Gel Transfer Device that performs western blotting transfer simply, efficiently, and reliably, within seven minutes without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane.

How the iBlot 2 System works
Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion unlike conventional protein transfer techniques (including wet, semi-wet, and semi-dry) which use inert electrodes that generate oxygen. With the iBlot 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer.

• Simple, user-friendly system
• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfers
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• Ability to create new custom programs
• Built-in tutorial and application notes