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Invitrogen™ TaqDNA-polymerase, nativ, m/W1
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Mængde:
100 Unit(s)
500 enheder
Pakningsstørrelse:
Hver
Beskrivelse
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The 1% solution of the detergent W-1 that is included may improve the thermostability of the enzyme when added at a final concentration of 0.05% (v/v).
Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:
Your choice of recombinant or native enzyme Amplification of PCR products up to 5 kb in size An enzyme that is licensed and qualified for PCR
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
Source
Native enzyme is purified from Thermus aquaticus YT1. Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
Source
Native enzyme is purified from Thermus aquaticus YT1. Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Tekniske data
Tekniske data
| Koncentration | 5 U/μL |
| Indhold og opbevaring | –20°C |
| Oversigt | Taq DNA Polymerase, 100 Unit, 5μL Concentration, Liquid, Colorless, 3'-A Overhang, Taq DNA Polymerase, 1X Low GC-Rich PCR Performance, Standard, Reaction Speed, Separate Components Reaction Format |
| Form | Liquid |
| Mængde | 100 Unit(s) |
| Til brug med (applikation) | Standard PCR |
| Overhæng | 3'-A |
| Inkluderer | Taq DNA polymerase (20μL), 10X PCR buffer without magnesium (1.25mL), 50mM Magnesium chloride (1mL), 1% W-1 (500μL) |
| Kilde | Thermus aquaticus YT1 |
Må kun bruges til undersøgelsesformål. Må ikke anvendes ved diagnostiske procedurer.
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