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Gibco™ Human VEGF-165 Recombinant Protein

Artikelnummer. 10111908
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Mængde:
1 mg
10 μg
100 μg
Pakningsstørrelse:
Hver
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Artikelnummer. 10111908

Brand: Gibco™ PHC9393

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Beskrivelse

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P15692
Til brug med (applikation) Bioactivity
Formulering Protein with no preservative
Gen-id (Entrez) 7422
Molekylvægt (g/mol) 40 kDa
Navn Human VEGF-165
Oprensningsmetode Purified
Mængde 1 mg
Opbevaringskrav 4°C
Regulatorisk status RUO
Endotoksin koncentration <0.1 ng/μg
Gene Alias H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
Fælles navn VEGF-165
Gen symbol Vegfa
Biologisk aktivitet ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
Produkttype Protein
Konjugeret Unconjugated
Arter Human
Rekombinant Recombinant
Sekvens APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Indhold og opbevaring 4°C
Klassifikation Carrier-Free
Aktivitet ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
Bioassay Proliferation of HUVECs (Human Umbilical Vein Endothelial Cells)
Endotoksin niveau <0.1 ng/μg
Forsendelsestilstand Wet Ice
Specifik aktivitet 1.0 x 106 - 1.7 x 105 units⁄mg
Udtrykssystem HEK293 cells
Protein familie Growth Factors & Receptors
Protein form Recombinant, Ligand
Form Lyophilized
Protein undertype VEGF (Vascular Endothelial Growth Factor)
Forskningskategori Oncology, Angiogenesis, Stem Cell Research, Differentiation, Inflammation
Produktlinje Gibco
Renhed eller kvalitetsklasse ≥95% by SDS-PAGE and HPLC
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