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Invitrogen™ Human PLEKHM3 (aa 71-158) Control Fragment Recombinant Protein
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Mængde:
100 μL
Beskrivelse
Highest antigen sequence indentity to the following orthologs: Mouse (93%), Rat (93%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57127 (PA5-57127. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.
Tekniske data
Tekniske data
| Adgangsnummer | Q6ZWE6 |
| Koncentration | ≥5.0 mg/mL |
| Til brug med (applikation) | Blocking Assay, Control |
| Formulering | 1 M urea, PBS with no preservative; pH 7.4 |
| Gen-id (Entrez) | 389072 |
| Navn | Human PLEKHM3 (aa 71-158) Control Fragment |
| Mængde | 100 μL |
| Regulatorisk status | RUO |
| Gene Alias | 9430067K14Rik; A230102O09Rik; AI449570; Dapr; differentiation associated protein; Differentiation-associated protein; PH domain-containing family M member 3; pleckstrin homology domain containing M3; pleckstrin homology domain containing, family M, member 1-like; pleckstrin homology domain containing, family M, member 3; pleckstrin homology domain-containing family M member 3; PLEKHM1L; Plekhm3; RGD1307151 |
| Fælles navn | PLEKHM3 |
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