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Proteintech™ Human P-Cadherin ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous P-cadherin

Brand:  Proteintech™ KE00117-96T


Product Code. 16851526

  • 2007.00 DKK / 96 tests

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Description

Description

  • Recognizes human P-cadherin
  • Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play role in maintenance of normal tissue architecture
    • P-cadherin (Placental cadherin, CDH3, cadherin-3) is a classical member of cadherin superfamily
    • Expression reported in many cancers (breast, gastric, endometrial, ovian and prostate)
    • Can be cleaved into soluble fragment, found in human  milk, semen, serum and NAF

Process:

  • Antibody specific for P-cadherin pre-coated onto microwells
  • P-cadherin protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for P-cadherin is added to detect captured P-cadherin protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

BC041846
0.5 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
4.5 hr.
31.25 to 2000 pg/mL
ISO 13485
Coated ELISA Kit
DAB
1001
Solid
<10%
-20°C to +4°C
P22223
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