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Gibco™ Human M-CSF Recombinant Protein Product Code.: 10089418

Gibco™ Human M-CSF Recombinant Protein

Product Code. p-5232812
10 μg, Each
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Quantity:
10 μg
100 μg
Unit Size:
Each
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Product Code. 10089418

Brand: Gibco™ PHC9504

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Recombinant Protein

Reconstitution: Lyophilized human M-CSF should be reconstituted in 20mM Tris-HCl, pH 8.0 to a concentration of 0.1-1.0 mg/mL. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: Lyophilized human M-CSF should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human M-CSF at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P09603
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 1435
Molecular Weight (g/mol) 38 kDa
Name Human M-CSF
Quantity 10 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias C87615; colony stimulating factor 1; colony stimulating factor 1 (macrophage); colony-stimulating factor-1 splice variant; CSF1; CSF-1; Csfm; H-MCSF; lanimostim; macrophage colony stimulating factor 1; macrophage colony stimulating factor alpha; macrophage colony stimulating factor beta; macrophage colony-stimulating factor; macrophage colony-stimulating factor 1; macrophage colony-stimulating factor beta; macrophage-colony stimulating factor alpha; MCSF; MCSF alpha; MCSFBETA; MGC31930; M-MCSF; op; osteopetrosis; Processed macrophage colony-stimulating factor 1
Common Name M-CSF
Biological Activity ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of M-NSF60 cells.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage 4°C
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Expression System HEK293 cells
Protein Family Cytokines & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype GM-CSF, G-CSF, & M-CSF
Research Category Cardiovascular Research, Stem Cell Research, Differentiation, Inflammation
Product Line Gibco™
Purity or Quality Grade >95% by SDS-PAGE
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