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  5. Invitrogen™ Human IL-12/IL-23 p40 ELISA Kit

Invitrogen™ Human IL-12/IL-23 p40 ELISA Kit

Artikelnummer. 15521487
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Mængde:
10 x 96 tests
96 prøver
Pakningsstørrelse:
96 tests
Pakke med 10
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15521487 96 prøver 96 tests
15541487 10 x 96 tests Pakke med 10
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Artikelnummer. 15521487 Leverandør Invitrogen™ Leverandørnr. BMS2013

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5073.00 DKK valid until 2026-06-30
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Beskrivelse

Indeholder

Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-12/IL-23 p40
Biotin-Conjugate anti-human IL-12/IL-23 p40 monoclonal antibody
Streptavidin-HRP
Human IL-12/IL-23 p40 Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

Human Interleukin-12/Interleukin-23 p40-underenhed (Hu IL-12/IL-23 p40) ELISA kvantificerer Hu IL-12/IL-23 p40 i humant serum, plasma, bufferopløsning eller cellekulturmedium. Assayet vil udelukkende genkende både naturligt og rekombinant Hu IL-12/IL-23 p40. Metodens princip The Human IL-12/IL-23 p40 solid-phase sandwich ELISA (enzym-linked immunosorbent assay) er designet til at måle mængden af målet bundet mellem et matchet antistofpar. Et målspecifikt antistof er blevet præcoatet i brøndene på den medfølgende mikroplade. Prøver, standarder eller kontroller tilsættes derefter til disse brønde og binder til det immobiliserede (fangende) antistof. Sandwichen dannes ved tilsætning af det andet (detektor) antistof, en substratopløsning tilsættes, der reagerer med enzym-antistof-målkomplekset for at producere et målbart signal. Intensiteten af dette signal er direkte proportional med koncentrationen af mål til stede i den originale prøve. Strenge validering Hvert fremstillet parti af dette ELISA kit er kvalitetstestet for kriterier som sensitivitet, specificitet, præcision og lot-to-lot konsistens. Se manualen for mere information om validering.

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P29459, P29460, Q9NPF7
Analyseområde 31,3 til 2.000 pg/ml
Assay følsomhed <10 pg/mL
Indhold og opbevaring Aluminum pouch(es) with a Microplate coated with polyclonal antibody to human IL-12/IL-23 p40
Biotin-Conjugate anti-human IL-12/IL-23 p40 monoclonal antibody
Streptavidin-HRP
Human IL-12/IL-23 p40 Standard lyophilized, 4 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Konjugeret HRP
Immunoassay-kitformat Sandwich ELISA Kit
Produkttype ELISA
Prøvetype Plasma, Serum, Supernatant
Målarter ADN
Til brug med (applikation) ELISA
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; Il12; Il-12 p35; IL12 p40; IL23; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL12p35; IL-12p35; Il12p40; Il-12p40; il23; IL-23; IL23 p41; IL-23 subunit alpha; Il23a; IL-23A; IL-23-A; IL23P19; IL-23p19; IL35 subunit; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 polypeptide 35; interleukin 12 subunit p40; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12b; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; Interleukin12; interleukin-12 alpha chain; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-23 p19 subunit; interleukin-23 p40 subunit; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; Ll12a; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p19; P35; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; Il12; Il-12 p35; IL12 p40; IL23; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL12p35; IL-12p35; Il12p40; Il-12p40; il23; IL-23; IL23 p41; IL-23 subunit alpha; Il23a; IL-23A; IL-23-A; IL23P19; IL-23p19; IL35 subunit; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 polypeptide 35; interleukin 12 subunit p40; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12b; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; Interleukin12; interleukin-12 alpha chain; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-23 p19 subunit; interleukin-23 p40 subunit; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; Ll12a; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p19; P35; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798; CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2;IL-23; IL-23A; IL23P19; P19; SGRF
Gen-id (Entrez) 3592, 3593, 51561
Gen symbol CLMF,CLMF2,IL-12 B,IMD28,IMD29,NKSF,NKSF2,IL-23,IL-23 A,IL23P19,P19,SGRF
Interassay CV 6.4%
Intraassay CV 4.7%
Sættets indhold Forbelagt 96-brønds plade, Standard, Prøve fortynder, Analysebufferkoncentrat, Biotinyleret detektionsantistof, SAV-HRP, Vaskebuffer, kromogen, Stop Løsning, Klæbende pladedæksler
Etiket eller farvestof Biotin
Mængde 96 prøver
Regulatorisk status RUO
Prøvevolumen Plasma 50μ L, Serum 50μ L, Supernatant 50μ L
Opbevaringskrav 2 °C til 8 °C
Synonym Interleukin-12 subunit beta precursor
Mål IL-12/IL-23 p40
Test tid 1 t 20 min
Samlet analysetid 4 timer 10 min
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