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IgM, Super Bright 436, Clone: SA-DA4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 62-9998-42

Additional Details : Weight : 0.01000kg

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Product Code. 15855543

  • 2138.00 DKK / Pack of 100


Estimated Shipment date
from Supplier 05-11-2018
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Description

Description

The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5μL (0.25μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL.Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 436 can be excited with the violet laser line (405nm) and emits at 436nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions.Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405nm; Emission: 436nm; Laser: Violet Laser
Specifications

Specifications

IgM
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P01871
IGHM
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
3507
Flow Cytometry
SA-DA4
Super Bright 436
IGHM
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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