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Invitrogen™ Human HMGB-1 ELISA Kit

Artikelnummer. 17906839
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Artikelnummer. 17906839 Leverandør Invitrogen™ Leverandørnr. EEL047
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Forventet forsendelse: 06-08-2026
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Beskrivelse

ELISA

The Human High Mobility Group Protein B1 (HMGB-1) ELISA quantitates HMGB-1 in serum, plasma, and other biological fluids The Human High Mobility Group Protein B1 (HMGB-1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P09429
Analyseområde 31,25 til 2000 pg/ml
Assay følsomhed 18.75 pg/mL
Konjugeret HRP
Immunoassay-kitformat Sandwich ELISA Kit
Produkttype ELISA
Protein familie High mobility group box
Prøvetype Plasma, Serum, Supernatant
Målarter Human
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias Ac2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein productAc2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product;
Gen-id (Entrez) 3146
Gen symbol Hmgb1
Interassay CV <10%
Intraassay CV <10%
Sættets indhold Forbelagt 96-brønds plade, Standard, Biotinyleret detektionsantistof, HRP konjugat, Standard& Prøve fortynder, Biotinyleret detektionsantistoffortynder, HRP konjugatfortynder, Vaskebuffer, TMB substrat, Stop Løsning, Pladeforsegler
Etiket eller farvestof Biotin
Mængde 96 prøver
Regulatorisk status RUO
Prøvevolumen Plasma 100μ L, Serum 100μ L, Supernatant 100μ L
Forsendelsestilstand Wet or Dry Ice
Opbevaringskrav Se produktdokumentationen for komponentspecifik opbevaringstemperatur.
Mål HMGB1
Test tid 1 t 20 min
Samlet analysetid 3 timer 30 min
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