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  5. Cytiva HiTrap™ TALON™ Crude Prepacked Columns

Cytiva HiTrap™ TALON™ Crude Prepacked Columns

Product Code. 12034118 Shop All Cytiva Products
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Product Code. Quantity Unit Size
12034118 5 x 5 mL Pack of 5
11618893 5 x 1 mL Pack of 5
15147946 1 x 1 mL 1mL
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Product Code. 12034118 Supplier Cytiva Supplier No. 28953767
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Beschreibung

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

TRUSTED_SUSTAINABILITY

Spezifikation

Quantity 5 x 5 mL
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Storage Requirements 4°C to 8°C
Binding Capacity Up to 20 mg Histidine-tagged Protein1
Diameter (English) Inner 0.62 in.
Product Type HiTrap Talon crude prepacked Column
Diameter (Metric) Inner 1.6 cm
Flow Rate 1 to 4 mL/min
Particle Size 60 to 160 μm
Material Polypropylene
Max. Pressure 3 bar
Matrix 6% Cross-Linked Agarose
pH Range 3 to 12
Storage Buffer 20% Ethanol
Ligand Type Talon Superflow Cobalt-IMAC
Packaging Quantity 5 x 5 mL
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Sicherheit und Handhabung

compliance-icons
Produktidentifikator
  • HiTrap TALON crude, 5 ml, 5 x 5 ml
 Signalwort
  • Warning
 Gefahrenkategorie
  • Flammable liquid Category 2
 Gefahrenhinweis
  • H225-Highly flammable liquid and vapour.
  • H226-Flammable liquid and vapour.
  • EUH208-Contains . May produce an allergic reaction.
 Sicherheitshinweis
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Ergänzende Informationen
  • MIXTURE LIST-Contain: ethanol, methanol, cobalt
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