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Cytiva HiTrap™ rProtein A FF Columns Product Code.: 11708548
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Cytiva HiTrap™ rProtein A FF Columns

Product Code. 11708548 Shop All Cytiva Products
5 x 5 mL, <20 mL/min., Pack of 5
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Quantity:
1 x 5 mL
2 x 1 mL
5 x 1 mL
5 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Unit Size:
Each
Pack of 2
Pack of 5
This item is not returnable. View return policy

Product Code. 11708548

Brand: Cytiva 17508002

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This item is not returnable. View return policy

Description

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
  • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
  • Average particle size: 90μm
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Available as 1mL and 5mL columns
Media
  • Ligand: Recombinant protein A (E.coli)
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: ∽50mg human IgG/mL
  • pH stability working range: 3 to 10

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Order Info

1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.

TRUSTED_SUSTAINABILITY

Specifications

Binding Capacity ≈50 mg Human IgG/mL
Flow Rate <20 mL/min.
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Content And Storage 4°C to 30°C, 20% Ethanol
Quantity 5 x 5 mL

Safety and Handling

compliance-icons
Product Identifier
  • HiTrap rProtein A FF, 5 x 5 ml
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
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