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Cytiva HisTrap™ HP Prepacked Columns
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Quantity:
1 mL
5 mL
5 x 1 mL
5 x 5 mL
Diameter (Metric) Inner:
7 mm
16 mm
Unit Size:
1mL
25mL
5mL
Description
- Prepacked with Ni Sepharose™ High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34ÎĽm
- pH stability working range: 3 to 12
1mL columns
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
5mL columns
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.

Specifications
Specifications
Bed Volume | 5 mL |
Diameter (English) Inner | 0.6 in. |
Diameter (Metric) Inner | 16 mm |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
Material | Polypropylene |
pH Range | 3 to 12 |
Support Type | 6% Highly Cross-linked Agarose |
Content And Storage | 4°C to 30°C |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
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Safety and Handling

Signal Word
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- EUH208-Contains . May produce an allergic reaction.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol, Nickel
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