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Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
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Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.
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Highlights:
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator
High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate
High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Large scale FPLC purification of polyhistidine tagged proteins

Specifications
Specifications
Content And Storage | Store at 4°C |
Form | Liquid Suspension |
Product Line | HisPur™ |
Quantity | 10 mL |
Target | His-Tagged Protein |
Type | Agarose |
Stationary Phase | Metal Chelate, Divalent Cobalt |
Format | Bottle |
Column Type | Affinity, Agarose Resin |
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