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Cytiva His SpinTrap™ Spin Columns

Product Code. p-3676457 Shop All Cytiva Products
Spin Column, Pack of 50
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Type:
Spin Column
Spin Column Kit
Unit Size:
1 L
Pack of 50
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750ÎĽg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34ÎĽm

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 100 ÎĽL
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Quantity 1 Pack His SpinTrap Spin Columns (50 x 100 ÎĽL)
Type Spin Column
Sample Volume 600 ÎĽl

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Safety and Handling

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Signal Word
  • Warning
 Hazard Category
  • Skin sensitiser Category 1
 Hazard Statement
  • H317-May cause an allergic skin reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : nicke, reaction massof:5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2Hisothiazol-3-one
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