MP Biomedicals HEV IgM ELISA 3.0 (CE) 96 wells

The wells of the polystyrene microplate strips are coated with highly conserved conformational ORF2 epitope. Human serum or plasma, diluted in diluent buffer, are incubated in these coated wells. HEV specific antibodies, if present, will bind to the antigens immobilized on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials and mouse monoclonal anti-human IgM antibodies labeled with horseradish peroxidase is added to the wells. This labeled antibody will bind to any antigen-antibody complexes previously formed and excess unbound labeled antibodies are removed by washing. A colourless substrate solution containing 3,3',5,5'-tetramethylbenzidine (TMB) is then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of acid. The intensity of the resulting yellow product is measured at 450 nm using a spectrophotometer and is proportional to the amount of antibodies present in the specimen.

• CE-marked
• 96 wells
• For professional use only
• For HEV antibodies detection