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Invitrogen™ Goat anti-Human IgG Fc Secondary Antibody, PE, eBioscience™

Artikelnummer. 15290371
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Mængde:
100 μg
Pakningsstørrelse:
100µg
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15290371 100 μg 100µg
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Artikelnummer. 15290371 Leverandør Invitrogen™ Leverandørnr. 12499882

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Beskrivelse

Goat Polyclonal Secondary Antibody

Description: This goat antibody reacts with the Fc fragment of heavy chains on human IgG but notwith the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA, or against nonimmunoglobulinserum proteins. This antibody has been tested to ensure minimalcross-reaction with bovine, horse, and mouse serum proteins, but the antibody may cross-react with immunoglobulins fromother species. Applications Reported: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been reported for use in flow cytometric analysis. Applications Tested: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been tested by flow cytometric analysis to detect purified human Fc gamma-tagged recombinant proteins. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Tekniske data

Antigen Human IgG Fc
Applikationer Flow Cytometry
Klassifikation Polyclonal
Koncentration 0.5 mg/mL
Konjugeret PE
Formulering PBS with 0.09% sodium azide; pH 7.2
Værtsarter Goat
Oprensningsmetode Affinity chromatography
Mængde 100 μg
Regulatorisk status RUO
Primær eller sekundær Secondary
Målarter Human
Indhold og opbevaring 4°C, store in dark, DO NOT FREEZE!
Produkttype Secondary Antibody
Form Liquid
Isotype Ig
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