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AcroBiosystems GMP Human IL-1 beta Protein

Artikelnummer. 31453799
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10 mg
50 μg
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31453799 10 mg
31451805 50 μg
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Artikelnummer. 31453799 Leverandør AcroBiosystems Leverandørnr. GMPILBH1610mg

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Beskrivelse

Recombinant Protein

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL1β shares 96 % amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1β.

Tekniske data

Adgangsnummer Uniprot
Karakterisering IL-1 beta Structure This protein carries no tag. The protein has a calculated MW of 17.5 kDa. The protein migrates as 18 kDa±3 kDa under reducing (R) condition (SDS-PAGE).
Format Powder-GMP
Formulering Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Kvalitetskontrol test <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Mængde 10 mg
Kilde GMP Human IL-1 beta Protein (GMP-ILBH16) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # ). Request for sequence
Biologisk aktivitet GMP Human IL-1 beta Protein (Cat. No. GMP-ILBH16) stimulates proliferation of D10.G4.1 cells. The specific activity of GMP Human IL-1 beta Protein is>5.00 x 10^7 IU/mg, which is calibrated against WHO International Standard INTERLEUKIN-1 BETA (IL-1ß, Human rDNA derived) (NIBSC code: 86/680) (QC tested).∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-1beta.∣MALDI-TOF analysis of GMP Human IL-1 beta Protein (Cat. No. GMP-ILBH16). The labeled peaks at 17372.1 Da and 17301.3 Da correspond to the calculated molecular masses, 17377 Da (aa 117-269) and 17306 Da (aa 118-269), respectively (Routinely tested).
Arter Human
Sterilitet Sterile
Indhold og opbevaring -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Endotoksin niveau Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Forsendelsestilstand This product is supplied and shipped with blue ice, please inquire the shipping cost.
Renhed eller kvalitetsklasse 0.95
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